8RTX

Crystal Structure of an Anti-idiotype Fab Fragment

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE MASSIF-1
Synchrotron site	ESRF
Beamline	MASSIF-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-10-05
Detector	DECTRIS PILATUS3 X 2M
Wavelength(s)	0.9655
Spacegroup name	P 31
Unit cell lengths	81.857, 81.857, 181.927
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	40.930 - 2.700
R-factor	0.2496
Rwork	0.247
R-free	0.26920
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.631
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.930	2.770
High resolution limit [Å]	2.700	2.700
Number of reflections	35497	2632
<I/σ(I)>	9.76
Completeness [%]	94.6
Redundancy	2.05
CC(1/2)	0.998	0.616

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	PEG 3350, ammonium sulfate, imidazole