8RTC
Rap from bacteriophage Phi3T in presence of pheromone RGHTS
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-09-24 |
| Detector | DECTRIS PILATUS3 X 6M |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 60.600, 92.660, 101.970 |
| Unit cell angles | 90.00, 107.07, 90.00 |
Refinement procedure
| Resolution | 97.480 - 2.830 |
| R-factor | 0.2301 |
| Rwork | 0.228 |
| R-free | 0.26720 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.772 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 97.480 | 2.931 |
| High resolution limit [Å] | 2.830 | 2.830 |
| Number of reflections | 24531 | 2392 |
| <I/σ(I)> | 23.93 | |
| Completeness [%] | 91.5 | |
| Redundancy | 21.6 | |
| CC(1/2) | 0.898 | 0.574 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 294 | 25% glycerol ethoxylate, 0.2M NH4Cl |
