8RT2

Hen Egg White Lysozyme Crystallized with Bioassembler on Earth

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-12-10
Detector	DECTRIS EIGER2 X CdTe 16M
Wavelength(s)	0.61992
Spacegroup name	P 43 21 2
Unit cell lengths	77.030, 77.030, 38.970
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	18.730 - 0.800
R-factor	0.1216
Rwork	0.121
R-free	0.13260
Structure solution method	SAD
RMSD bond length	0.011
RMSD bond angle	1.240
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.970	0.820
High resolution limit [Å]	0.800	0.800
Number of reflections	236365	17476
<I/σ(I)>	15.54
Completeness [%]	99.9
Redundancy	6.6
CC(1/2)	0.999	0.247

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	4.8	294	SODIUM ACETATE 0.1M AT PH 4.8, NACL 13%, Gd(HP-D03A) 250mM, 294K