8RST
Rap from bacteriophage Phi3T in presence of pheromone SRGHTS
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALBA BEAMLINE XALOC |
Synchrotron site | ALBA |
Beamline | XALOC |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-03-12 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9792 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 60.843, 195.549, 92.441 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.890 - 2.560 |
R-factor | 0.2112 |
Rwork | 0.209 |
R-free | 0.25530 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.002 |
RMSD bond angle | 0.382 |
Data reduction software | MOSFLM |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.890 | 2.655 |
High resolution limit [Å] | 2.560 | 2.560 |
Number of reflections | 18149 | 1784 |
<I/σ(I)> | 20.73 | |
Completeness [%] | 99.9 | |
Redundancy | 8 | |
CC(1/2) | 1.000 | 0.649 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 294 | 0.1M Tris pH8.5, 15% glycerol ethoxylate, 4% tetrahydrofuran |