8RSN
macrodomain-fused SirTM (Mfs1) from Fusarium oxysporum f. sp. cubense race 1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-06-29 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.28229 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 61.739, 135.089, 147.625 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 99.859 - 2.219 |
| Rwork | 0.203 |
| R-free | 0.25320 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.751 |
| Data reduction software | DIALS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0415) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 147.600 | 2.280 |
| High resolution limit [Å] | 2.219 | 2.220 |
| Number of reflections | 61954 | 4501 |
| <I/σ(I)> | 13.2 | 1.4 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 11.9 | 6.9 |
| CC(1/2) | 0.999 | 0.512 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 200 mM KSC 100 mM BisTris-propane pH 7.5 20% (w/v) PEG3350 |
