8RR3

Human mitochondrial RNase Z complex with ELAC2-D550N catalytic mutant and tRNA-Gln precursor (Composite model)

EM specimen

Vitrification	Yes
Instrument	FEI VITROBOT MARK IV

EM Data Collection

Microscope	FEI TITAN KRIOS
Electron Gun	FIELD EMISSION GUN
Accelerating Voltage (kV)	300
Detector	GATAN K3 (6k x 4k)

3D Reconstruction

Method	SINGLE PARTICLE
Resolution (Å)	3.4
Number of Particle	12168828
Software	Warp,ISOLDE,PHENIX,cryoSPARC,SerialEM,DigitalMicrograph,UCSF ChimeraX,Coot
CTF correction	PHASE FLIPPING AND AMPLITUDE CORRECTION