Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-03-01 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 43 |
| Unit cell lengths | 114.618, 114.618, 48.882 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.900 - 2.900 |
| R-factor | 0.222 |
| Rwork | 0.220 |
| R-free | 0.26580 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.205 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.900 | 3.090 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.249 | |
| Rpim | 0.084 | |
| Number of reflections | 14355 | 2300 |
| <I/σ(I)> | 9 | |
| Completeness [%] | 99.8 | |
| Redundancy | 10.2 | |
| CC(1/2) | 0.947 | 0.347 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 288 | 0.02M Divalent II, 0.1M buffer system 6, pH 8.5, 30% precipitant mix 7 (Morpheus II screen B9) |
