8RQT

Crystal structure of Pichia pastoris Pex8

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-12-16
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.9762
Spacegroup name	P 21 21 21
Unit cell lengths	81.149, 87.842, 153.093
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.030 - 2.410
Rwork	0.212
R-free	0.25810
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	1.582
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	AutoSol
Refinement software	REFMAC (5.8.0425)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	47.030	47.030	2.500
High resolution limit [Å]	2.410	9.020	2.410
Rmerge	0.188	0.035	4.834
Rmeas	0.204	0.038	5.212
Rpim	0.077	0.015	1.943
Number of reflections	43103	938	4486
<I/σ(I)>	10
Completeness [%]	100.0	100
Redundancy	13.2	10.8	13.8
CC(1/2)	0.999	0.999	0.415

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	292	0.09M NPS, 0.1M pH 7.5 (Sodium HEPES + MOPS), 12% Ethylene glycol, 6% w/v PEG 8000, 4% Formamide