8RQR
In meso structure of apolipoprotein N-acyltransferase, Lnt, from Escherichia coli in 7.10 monoacylglycerol
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I24 |
Synchrotron site | Diamond |
Beamline | I24 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-12-10 |
Detector | DECTRIS EIGER2 X CdTe 9M |
Wavelength(s) | 0.9686 |
Spacegroup name | P 21 2 21 |
Unit cell lengths | 48.679, 76.461, 157.080 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.500 - 2.190 |
R-factor | 0.2009 |
Rwork | 0.199 |
R-free | 0.24580 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.004 |
RMSD bond angle | 0.798 |
Data reduction software | XDS |
Data scaling software | STARANISO |
Phasing software | PHASER |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.500 | 2.270 |
High resolution limit [Å] | 2.190 | 2.190 |
Number of reflections | 30881 | 2997 |
<I/σ(I)> | 7.7 | |
Completeness [%] | 99.1 | |
Redundancy | 6.4 | |
CC(1/2) | 0.990 | 0.310 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | LIPIDIC CUBIC PHASE | 293 | 100 mM MES pH 6.0, 8 %(v/v) MPD, and 50-400 mM sodium or potassium thiocyanate |