8RQP
In meso structure of the alginate exporter, AlgE, from Pseudomonas aeruginosa in 7.10 monoacylglycerol
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-12-10 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9779 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 57.358, 74.546, 115.913 |
Unit cell angles | 90.00, 102.49, 90.00 |
Refinement procedure
Resolution | 39.370 - 1.450 |
R-factor | 0.1915 |
Rwork | 0.190 |
R-free | 0.22290 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.012 |
RMSD bond angle | 1.272 |
Data reduction software | XDS |
Data scaling software | STARANISO |
Phasing software | PHASER |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.400 | 1.500 |
High resolution limit [Å] | 1.450 | 1.450 |
Number of reflections | 61350 | 3068 |
<I/σ(I)> | 7.2 | |
Completeness [%] | 90.7 | |
Redundancy | 6.8 | |
CC(1/2) | 0.990 | 0.273 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | LIPIDIC CUBIC PHASE | 293 | 34-41 %(v/v) PEG400, 100 mM LiSO4, and 100 mM Na-Citrate at pH 5.6 and 6.0 |