8RQP
In meso structure of the alginate exporter, AlgE, from Pseudomonas aeruginosa in 7.10 monoacylglycerol
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-12-10 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9779 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 57.358, 74.546, 115.913 |
| Unit cell angles | 90.00, 102.49, 90.00 |
Refinement procedure
| Resolution | 39.370 - 1.450 |
| R-factor | 0.1915 |
| Rwork | 0.190 |
| R-free | 0.22290 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.272 |
| Data reduction software | XDS |
| Data scaling software | STARANISO |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.400 | 1.500 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Number of reflections | 61350 | 3068 |
| <I/σ(I)> | 7.2 | |
| Completeness [%] | 90.7 | |
| Redundancy | 6.8 | |
| CC(1/2) | 0.990 | 0.273 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 293 | 34-41 %(v/v) PEG400, 100 mM LiSO4, and 100 mM Na-Citrate at pH 5.6 and 6.0 |
