8RPG
Crystal structure of an alcohol oxidase from Streptomyces hiroshimensis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-10-26 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.979180 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 80.510, 97.688, 68.047 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 62.210 - 2.000 |
| R-factor | 0.15317 |
| Rwork | 0.151 |
| R-free | 0.19324 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.651 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 97.690 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.057 | 0.235 |
| Number of reflections | 36978 | 5325 |
| <I/σ(I)> | 15.4 | 5.3 |
| Completeness [%] | 100.0 | |
| Redundancy | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 291.15 | 30 % Polyethylene glycol 4000 100 mM TRIS pH 8.5 200 mM Magnesium chloride |
