8RON
Crystal structure of human FAD synthase, isoform 2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-11-10 |
| Detector | DECTRIS PILATUS3 2M |
| Wavelength(s) | 0.8731 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 75.754, 187.740, 79.383 |
| Unit cell angles | 90.00, 96.45, 90.00 |
Refinement procedure
| Resolution | 58.720 - 2.600 |
| R-factor | 0.2181 |
| Rwork | 0.215 |
| R-free | 0.26830 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.825 |
| Data reduction software | autoPROC |
| Data scaling software | autoPROC |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19_4092) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 58.725 | 2.792 |
| High resolution limit [Å] | 2.488 | 2.488 |
| Number of reflections | 43831 | 2192 |
| <I/σ(I)> | 9.8 | 1.5 |
| Completeness [%] | 93.2 | 71.3 |
| Redundancy | 6.6 | 7 |
| CC(1/2) | 0.996 | 0.485 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 0.1 M MES pH 6.5, 10% PEG 8000 and 10% ethylene glycol |
