8ROM
Crystal structure of human FAD synthase PAPS domain in complex with FAD
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2022-06-23 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9919 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 63.959, 69.731, 105.330 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 31.360 - 1.690 |
| R-factor | 0.1828 |
| Rwork | 0.181 |
| R-free | 0.20660 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.192 |
| Data reduction software | autoPROC |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19_4092) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 58.144 | 1.720 |
| High resolution limit [Å] | 1.690 | 1.690 |
| Number of reflections | 24610 | 1321 |
| <I/σ(I)> | 8.3 | 2.3 |
| Completeness [%] | 92.1 | 99.7 |
| Redundancy | 4.3 | 4.2 |
| CC(1/2) | 0.996 | 0.649 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293 | 1.6 M Na/K phosphate, pH 6, 10% DMSO |
