8ROI
Human cohesin SMC3-HD(EQ)/RAD21-N complex - ADP-bound conformation
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-05-05 |
Detector | DECTRIS PILATUS 2M-F |
Wavelength(s) | 1.000008 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 89.949, 89.949, 233.913 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.030 - 2.450 |
R-factor | 0.1969 |
Rwork | 0.195 |
R-free | 0.23880 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 0.940 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHENIX |
Refinement software | PHENIX (1.17_3644) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.600 |
High resolution limit [Å] | 2.450 | 2.450 |
Rmerge | 0.154 | 2.621 |
Number of reflections | 36214 | 5698 |
<I/σ(I)> | 22.6 | 1.5 |
Completeness [%] | 99.9 | 99.7 |
Redundancy | 26.1 | 26.4 |
CC(1/2) | 0.999 | 0.581 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M Na/K phosphate pH 7.5; 0.1 M HEPES pH 7.5; 15% v/v PEG Smear High; 10% v/v ethylene glycol |