8ROA
Human cohesin SMC1A-HD(longCC-EQ)/RAD21-C complex - ADP-bound conformation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 2 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-05-25 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.979950 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 189.311, 65.278, 105.762 |
| Unit cell angles | 90.00, 116.66, 90.00 |
Refinement procedure
| Resolution | 47.809 - 2.436 |
| R-factor | 0.1969 |
| Rwork | 0.194 |
| R-free | 0.24970 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.965 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.580 |
| High resolution limit [Å] | 2.436 | 2.440 |
| Rmerge | 0.120 | 1.121 |
| Number of reflections | 43374 | 6862 |
| <I/σ(I)> | 11.6 | 1.26 |
| Completeness [%] | 99.5 | 98.3 |
| Redundancy | 6.4 | 5.2 |
| CC(1/2) | 0.998 | 0.579 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2 M ammonium formate; 20% w/v PEG 3350 |
