8RO5
HLA-A3 restricted mG12V-TCR
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-08-17 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 68.575, 43.320, 84.243 |
| Unit cell angles | 90.00, 108.50, 90.00 |
Refinement procedure
| Resolution | 40.200 - 1.700 |
| R-factor | 0.1843 |
| Rwork | 0.183 |
| R-free | 0.20930 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.995 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.200 | 1.762 |
| High resolution limit [Å] | 1.700 | 1.701 |
| Number of reflections | 51656 | 5022 |
| <I/σ(I)> | 16.88 | 3.1 |
| Completeness [%] | 99.1 | |
| Redundancy | 1.05 | |
| CC(1/2) | 1.000 | 1.000 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 298 | n |
