8RNH

Crystal structure of HLA B*18:01 in complex with EEIEITTHF, an 9-mer epitope from Influenza A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-07-30
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.953732
Spacegroup name	P 21 21 21
Unit cell lengths	50.750, 81.627, 110.556
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.120 - 1.600
R-factor	0.2033
Rwork	0.202
R-free	0.23450
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.916
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.120	1.630
High resolution limit [Å]	1.600	1.600
Number of reflections	59129	3078
<I/σ(I)>	10.3	1.6
Completeness [%]	97.0
Redundancy	4.7
CC(1/2)	0.997	0.630

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2 M Lithium Acetate, 20% w/v PEG 3350, and 10 mM MgCl2