8RNF
HLA-E*01:03 in complex with SARS-CoV-2 Omicron Nsp13 peptide, VIPLSAPTL
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX IV BEAMLINE BioMAX |
| Synchrotron site | MAX IV |
| Beamline | BioMAX |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-06-09 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97625 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 122.233, 66.530, 158.446 |
| Unit cell angles | 90.00, 95.26, 90.00 |
Refinement procedure
| Resolution | 47.950 - 1.872 |
| Rwork | 0.178 |
| R-free | 0.21970 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.434 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.950 | 1.900 |
| High resolution limit [Å] | 1.870 | 1.870 |
| Rmerge | 0.120 | 0.847 |
| Rmeas | 0.142 | |
| Rpim | 0.525 | |
| Number of reflections | 104376 | 4978 |
| <I/σ(I)> | 10.5 | 2.3 |
| Completeness [%] | 99.8 | |
| Redundancy | 2 | |
| CC(1/2) | 0.997 | 0.730 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 0.15 M DL malic acid and 20% w/v of PEG3350 |
