8RMJ
Drosophila Semaphorin 2b in complex with glycosaminoglycan mimic SOS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-08-03 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 181.489, 181.489, 168.379 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 45.670 - 2.790 |
| R-factor | 0.2478 |
| Rwork | 0.246 |
| R-free | 0.27380 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.546 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19_4092) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 157.170 | 2.820 |
| High resolution limit [Å] | 2.790 | 2.790 |
| Rmeas | 0.086 | |
| Number of reflections | 23325 | 1961 |
| <I/σ(I)> | 21.6 | |
| Completeness [%] | 96.4 | 100 |
| Redundancy | 19.6 | 16.1 |
| CC(1/2) | 1.000 | 0.400 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293 | 0.1 M MES pH 6.0, 5% PEG 6000 |
