8RM2
Erk2 MAP kinase T188E mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-09-04 |
| Detector | DECTRIS PILATUS3 2M |
| Wavelength(s) | 0.965 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 48.306, 68.880, 59.705 |
| Unit cell angles | 90.00, 109.02, 90.00 |
Refinement procedure
| Resolution | 43.660 - 1.550 |
| R-factor | 0.20209 |
| Rwork | 0.200 |
| R-free | 0.23639 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.751 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.660 | 1.580 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Number of reflections | 53453 | 2597 |
| <I/σ(I)> | 9 | |
| Completeness [%] | 95.6 | |
| Redundancy | 3.2 | |
| CC(1/2) | 0.995 | 0.612 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 0.2M ammonium sulfate, 0.1M MES monohydrate pH=6.5 and 30% w/v PEG monomethylether 5,000 |
