8RKP
Cytochrome c prime from Hydrogenophilus thermoluteolus: Ferrous recombinant native with bound NO
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-04-22 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 62 2 2 |
| Unit cell lengths | 83.671, 83.671, 88.247 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 72.570 - 1.630 |
| R-factor | 0.19835 |
| Rwork | 0.197 |
| R-free | 0.21928 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 2.431 |
| Data reduction software | DIALS |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 72.570 | 1.660 |
| High resolution limit [Å] | 1.630 | 1.630 |
| Rmerge | 0.117 | 4.255 |
| Rmeas | 0.120 | 4.362 |
| Rpim | 0.027 | 0.952 |
| Number of reflections | 23390 | 1135 |
| <I/σ(I)> | 13.4 | 0.4 |
| Completeness [%] | 100.0 | 99.9 |
| Redundancy | 19.7 | 20.5 |
| CC(1/2) | 0.999 | 0.300 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 293 | 0.1 M sodium acetate pH 4.5, 0.2 M lithium sulphate, 30% (w/v) PEG 8,000 in a 96-well in-situ plate |
