8RKH

Crystal structure of the ZP-N2 and ZP-N3 domains of mouse ZP2 (mZP2-N2N3)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-05-22
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.91942
Spacegroup name	C 1 2 1
Unit cell lengths	163.010, 54.170, 68.660
Unit cell angles	90.00, 107.98, 90.00

Refinement procedure

Resolution	43.730 - 1.900
R-factor	0.2157
Rwork	0.214
R-free	0.24790
Structure solution method	SAD
RMSD bond length	0.005
RMSD bond angle	0.790
Data reduction software	XDS (Nov 1, 2016 BUILT=20161205)
Data scaling software	XSCALE (Nov 1, 2016 BUILT=20161205)
Phasing software	AutoSol (1.13_2998)
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.730	1.980
High resolution limit [Å]	1.900	1.900
Rmerge	0.065	2.330
Rmeas	0.072	2.572
Rpim	0.031	1.074
Number of reflections	44849	4926
<I/σ(I)>	12.6	0.8
Completeness [%]	99.2	98.5
Redundancy	5.2	5.4
CC(1/2)	1.000	0.490

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	20% (w/v) PEG 3350, 0.2 M Na-nitrate