8RKE
Crystal structure of the complete N-terminal region of human ZP2 (hZP2-N1N2N3)
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-06-13 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.91840 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 147.680, 97.770, 104.330 |
| Unit cell angles | 90.00, 94.80, 90.00 |
Refinement procedure
| Resolution | 21.920 - 2.700 |
| R-factor | 0.2297 |
| Rwork | 0.229 |
| R-free | 0.25780 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.775 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHARP |
| Refinement software | PHENIX (1.19.2_4158+SVN) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 21.920 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.082 | 2.152 |
| Rmeas | 0.089 | 2.345 |
| Rpim | 0.034 | 0.916 |
| Number of reflections | 40494 | 3976 |
| <I/σ(I)> | 15.7 | 0.9 |
| Completeness [%] | 99.2 | 98 |
| Redundancy | 6.9 | 6.4 |
| CC(1/2) | 1.000 | 0.510 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.5 | 277 | 10% (w/v) PEG 4000, 0.1 M MES/imidazole pH 6.5, 0.1 M carboxylic acids (MD2-100-76, Molecular Dimensions), 20% (v/v) glycerol |
