8RIX
Crystal structure of chloroplast FtsY from Arabidopsis thaliana in complex with the alarmone ppGpp
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-06-05 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.976253 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 138.280, 37.070, 59.820 |
| Unit cell angles | 90.00, 110.63, 90.00 |
Refinement procedure
| Resolution | 35.640 - 1.990 |
| R-factor | 0.2221 |
| Rwork | 0.219 |
| R-free | 0.27580 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.258 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.640 | 2.061 |
| High resolution limit [Å] | 1.990 | 1.990 |
| Rmerge | 0.118 | 0.547 |
| Rmeas | 0.128 | 0.606 |
| Rpim | 0.051 | 0.255 |
| Number of reflections | 19770 | 1978 |
| <I/σ(I)> | 14.86 | 2.95 |
| Completeness [%] | 99.6 | 99.5 |
| Redundancy | 6.3 | 5.5 |
| CC(1/2) | 0.996 | 0.917 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 200 mM Tris-HCl pH 8.5; 25% PEG1000 |
