8RIT

Dimeric mutant S103D of the BTB domain of ZBTB8A from Xenopus laevis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-11-25
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.98012
Spacegroup name	C 1 2 1
Unit cell lengths	259.993, 61.993, 71.488
Unit cell angles	90.00, 91.52, 90.00

Refinement procedure

Resolution	71.460 - 3.750
R-factor	0.2464
Rwork	0.244
R-free	0.29540
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.001
RMSD bond angle	0.307
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	129.960	3.810
High resolution limit [Å]	3.750	3.750
Rpim	0.129	0.777
Number of reflections	11929	547
<I/σ(I)>	4.5	1.2
Completeness [%]	99.7	93.7
Redundancy	6.5
CC(1/2)	0.914	0.798

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	MDL MORPHEUS I 1.38 (Alcohols, Buffer 1, EDO_P8K)