8RIR

S103A mutant of the BTB domain of ZBTB8A from Xenopus laevis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-11-25
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.98012
Spacegroup name	P 41 3 2
Unit cell lengths	154.149, 154.149, 154.149
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.500 - 3.720
R-factor	0.2634
Rwork	0.261
R-free	0.28970
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.001
RMSD bond angle	0.318
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.750	4.160
High resolution limit [Å]	3.720	3.720
Rpim	0.047	0.411
Number of reflections	7053	1944
<I/σ(I)>	10.4	2
Completeness [%]	99.9	100
Redundancy	19.1	20.1
CC(1/2)	0.999	0.786

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	caesium chloride, MES, Jeffamine M-600