8RIC

Crystallographic structure of oligosaccharide dehydrogenase from Pycnoporus cinnabarinus bound to Sinapic Acid, tetragonal crystal

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-09-15
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.98011
Spacegroup name	P 42 21 2
Unit cell lengths	147.531, 147.531, 142.790
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.040 - 1.860
Rwork	0.169
R-free	0.20200
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.015
RMSD bond angle	2.049
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0405)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.050	1.890
High resolution limit [Å]	1.860	1.860
Rpim	0.097	1.789
Number of reflections	131815	6475
<I/σ(I)>	8.4	0.8
Completeness [%]	100.0	100
Redundancy	27.6	28.5
CC(1/2)	0.996	0.336

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		294	2 M AMMONIUM SULFATE