8RHO

[2Fe:2S] ferredoxin FeSII from Azotobacter vinelandii, reduced form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM07
Synchrotron site	ESRF
Beamline	BM07
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-02-15
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9795
Spacegroup name	P 41 21 2
Unit cell lengths	60.955, 60.955, 139.414
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.100 - 1.450
R-factor	0.1537
Rwork	0.152
R-free	0.18160
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.471
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.470	1.470
High resolution limit [Å]	1.450	1.450
Number of reflections	88800	2280
<I/σ(I)>	28.5
Completeness [%]	100.0
Redundancy	23.1
CC(1/2)	1.000	0.809

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		297	50 mM glycine at pH 9 and 55 % (v/v) PEG400