8RHL
Yeast 20S proteasome in complex with a linear biarylether epoxyketone (compound 15a)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-05-05 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 135.210, 303.140, 143.230 |
Unit cell angles | 90.00, 113.09, 90.00 |
Refinement procedure
Resolution | 30.000 - 3.200 |
R-factor | 0.18639 |
Rwork | 0.184 |
R-free | 0.22770 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.002 |
RMSD bond angle | 1.193 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | REFMAC |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 3.300 |
High resolution limit [Å] | 3.200 | 3.200 |
Rmerge | 0.091 | 0.568 |
Number of reflections | 169944 | 13226 |
<I/σ(I)> | 10.2 | 2.2 |
Completeness [%] | 97.6 | |
Redundancy | 3.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 293 | 20 mM MgAC2, 13% MPD, 0.1 M MES |