8RGF

Arginase 2 in complex with inhibitor

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID30B
Synchrotron site	ESRF
Beamline	ID30B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-10-26
Detector	DECTRIS PILATUS 12M
Wavelength(s)	0.976
Spacegroup name	P 21 21 21
Unit cell lengths	46.940, 135.160, 145.150
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	98.916 - 1.860
R-factor	0.24
Rwork	0.239
R-free	0.26000
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.080
Data scaling software	Aimless
Refinement software	BUSTER (2.11.7 PACIOREK)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	98.916	1.888
High resolution limit [Å]	1.856	1.856
Rmerge	0.060	0.616
Rmeas	0.065	0.673
Rpim	0.026	0.267
Total number of observations	498259	24185
Number of reflections	78897	3869
<I/σ(I)>	14.2	2.4
Completeness [%]	99.7
Redundancy	6.3	6.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	13-18% PEG3350, 0.2-0.35M Am. Cit. pH7.0