8REK

Plasmodium vivax Apical Membrane Antigen 1/Fab complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-1
Synchrotron site	ESRF
Beamline	ID14-1
Temperature [K]	100
Detector technology	CCD
Collection date	2006-09-27
Detector	ADSC QUANTUM 210r
Wavelength(s)	0.934
Spacegroup name	P 21 21 2
Unit cell lengths	73.390, 214.680, 57.410
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	69.540 - 3.050
R-factor	0.22508
Rwork	0.221
R-free	0.29202
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.021
RMSD bond angle	1.646
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0415)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	73.390	3.260
High resolution limit [Å]	3.050	3.050
Rmerge	0.167	1.203
Rmeas	0.181	1.296
Rpim	0.067	0.480
Total number of observations	128197	23088
Number of reflections	18062	3193
<I/σ(I)>	11.8	1.7
Completeness [%]	99.9
Redundancy	7.1	7.2
CC(1/2)	0.996	0.316

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	290	Crystals were obtained by mixing 0.8 micro-L of PvAMA1-Fab complex with 0.2 micro-L of 15% 1,2,3 heptanetriol and 0.8 micro-L of reservoir buffer comprising 20% PEG 2000 monomethylether and 0.1 M Tris pH 7. The final protein concentration was 3.2 mg/ml.