8REH

Lysozyme measured via serial crystallography from a kapton HARE-chip (125 micron)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P14 (MX2)
Temperature [K]	293.15
Detector technology	PIXEL
Collection date	2023-05-04
Detector	DECTRIS EIGER X 4M
Wavelength(s)	0.976
Spacegroup name	P 43 21 2
Unit cell lengths	79.000, 79.000, 38.000
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	55.860 - 1.700
R-factor	0.1685
Rwork	0.168
R-free	0.17530
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.908
Data reduction software	CrystFEL
Data scaling software	CrystFEL
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	55.860	1.761
High resolution limit [Å]	1.380	1.700
Number of reflections	15648	1349
<I/σ(I)>	12.02	8.84
Completeness [%]	95.2	100
Redundancy	657.8	347.7
CC(1/2)	0.986	0.975

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	BATCH MODE		293.15	100mg/ml NaCl