8RDU

Conformational Landscape of the Type V-K CRISPR-associated TransposonIntegration Assembly CAST V-K composite map

EM specimen

Vitrification

Yes

EM Data Collection

Microscope	FEI TITAN KRIOS
Electron Gun	FIELD EMISSION GUN
Accelerating Voltage (kV)	300
Energy Filter	TFS Selectris X
Detector	FEI FALCON IV (4k x 4k)

3D Reconstruction

Method	SINGLE PARTICLE
Resolution (Å)	2.3
Software	cryoSPARC,PHENIX,ISOLDE,Coot,EPU,UCSF ChimeraX
CTF correction	PHASE FLIPPING AND AMPLITUDE CORRECTION