8RDP

Cereblon isoform 4 from Magnetospirillum gryphiswaldense in complex with spiro-isoxazol based compound 8a

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-06-11
Detector	DECTRIS EIGER2 S 16M
Wavelength(s)	1.0001
Spacegroup name	P 21 21 21
Unit cell lengths	56.356, 59.597, 87.875
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.480 - 1.760
R-factor	0.18771
Rwork	0.186
R-free	0.23045
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.012
RMSD bond angle	1.728
Data reduction software	XDS
Refinement software	REFMAC (5.8.0266)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	47.480	47.480	1.860
High resolution limit [Å]	1.760	5.240	1.760
Rmeas	0.130	0.130	0.130
Number of reflections	56873	2143	9222
<I/σ(I)>	8.06	24.74	1.04
Completeness [%]	100.0	99.9	99.8
Redundancy	6.89	7.06	6.4
CC(1/2)	0.997	0.997	0.562

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.4-0.6 M (NH4)H2PO4, ~17 mg/ml MSCI4 with 3mM thalidomide