8RDD
Crystal structure of Saccharomyces cerevisiae Nmd4 protein involved in nonsense mediated mRNA decay
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 2 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-04-14 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 80.371, 80.371, 94.134 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.190 - 1.796 |
| R-factor | 0.1816 |
| Rwork | 0.181 |
| R-free | 0.19160 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.860 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.200 | 1.853 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rpim | 0.002 | 0.580 |
| Number of reflections | 31899 | 1598 |
| <I/σ(I)> | 25.7 | 1.3 |
| Completeness [%] | 95.7 | |
| Redundancy | 20.1 | |
| CC(1/2) | 0.999 | 0.608 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 277 | 1M Na citrate, 0.1 M Na cacodylate pH 6.5, 0.2 M lithium acetate |
