8RCQ

Structural flexibility of Nucleoprotein of the Toscana virus in the presence of a nanobody.

Replaces:  8BPK

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	80
Detector technology	PIXEL
Collection date	2015-03-01
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9801
Spacegroup name	P 41 21 2
Unit cell lengths	54.755, 54.755, 380.647
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.460 - 3.800
Rwork	0.300
R-free	0.35000
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.597
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX ((1.19.2_4158: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.460	3.970
High resolution limit [Å]	3.800	3.800
Rmerge	0.200	1.800
Rmeas	0.210
Rpim	0.060
Number of reflections	6413	6413
<I/σ(I)>	12
Completeness [%]	99.5
Redundancy	10.9
CC(1/2)	0.999	0.780

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.5M MES, pH 6.5, 25% w/v PEG 2000