Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-03-08 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.9762 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 95.728, 38.562, 183.557 |
| Unit cell angles | 90.00, 104.66, 90.00 |
Refinement procedure
| Resolution | 74.150 - 1.900 |
| R-factor | 0.2265 |
| Rwork | 0.224 |
| R-free | 0.26880 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.992 |
| Data reduction software | DIALS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 88.790 | 1.930 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 102879 | 4432 |
| <I/σ(I)> | 7.5 | |
| Completeness [%] | 98.7 | |
| Redundancy | 5.1 | |
| CC(1/2) | 0.996 | 0.599 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 295 | 25% PEG3350, 25mM Sodium Phosphate pH 7.0, Streak Seeding |
