8RC7
The structure of membrane-active antibiotic cyclodecapeptide gramicidin S in complex with urea
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Temperature [K] | 293 |
Detector technology | DIFFRACTOMETER |
Collection date | 1978-01-01 |
Detector | CUSTOM-MADE |
Wavelength(s) | 1.5406 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 25.800, 25.800, 21.490 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 22.340 - 0.980 |
Rwork | 0.140 |
R-free | 0.13180 |
Structure solution method | AB INITIO PHASING |
RMSD bond length | 0.021 |
RMSD bond angle | 2.196 |
Refinement software | REFMAC (5.8.0411) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 22.340 |
High resolution limit [Å] | 0.980 |
Number of reflections | 4028 |
<I/σ(I)> | 210 |
Completeness [%] | 81.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Urea, HCl, 95% Ethanol |