8RAV
The crystal structure of DNA-bound human MutSbeta (MSH2/MSH3) in the canonical mismatch bound conformation with ADP bound in MSH2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-02-13 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.99989 |
| Spacegroup name | P 1 |
| Unit cell lengths | 66.599, 91.120, 96.482 |
| Unit cell angles | 67.56, 86.81, 74.64 |
Refinement procedure
| Resolution | 44.940 - 2.630 |
| R-factor | 0.23965 |
| Rwork | 0.239 |
| R-free | 0.28409 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 1.272 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.940 | 2.880 |
| High resolution limit [Å] | 2.630 | 2.630 |
| Number of reflections | 57658 | 13737 |
| <I/σ(I)> | 14.81 | |
| Completeness [%] | 95.9 | |
| Redundancy | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 277 | 0.1 M MES pH 6.5 -7.5, 0.2 M Ammonium Acetate, 20 - 25 % PEG 3350 |
