8RA0
Crystal structure of CysF
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-02-05 |
| Detector | DECTRIS EIGER2 X 16M |
| Wavelength(s) | 0.954 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 44.052, 68.776, 75.399 |
| Unit cell angles | 90.00, 96.83, 90.00 |
Refinement procedure
| Resolution | 50.650 - 1.890 |
| R-factor | 0.195 |
| Rwork | 0.193 |
| R-free | 0.23760 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 0.982 |
| Data reduction software | DIALS |
| Data scaling software | DIALS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21_5207) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.650 | 1.958 |
| High resolution limit [Å] | 1.890 | 1.890 |
| Rpim | 0.099 | 0.830 |
| Number of reflections | 35828 | 3488 |
| <I/σ(I)> | 5.97 | 0.69 |
| Completeness [%] | 99.6 | 97.34 |
| Redundancy | 6.9 | 7 |
| CC(1/2) | 0.992 | 0.419 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | JCSG Plus A2 0.1 M Sodium citrate 5.5 20 % w/v PEG 3000 |
