8R9B
A soakable crystal form of human CDK7 in complex with AMP-PNP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-11-19 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.99987 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 76.140, 180.285, 76.335 |
| Unit cell angles | 90.00, 117.85, 90.00 |
Refinement procedure
| Resolution | 90.140 - 2.210 |
| R-factor | 0.19491 |
| Rwork | 0.192 |
| R-free | 0.24265 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.547 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0232) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 90.140 | 2.391 |
| High resolution limit [Å] | 2.210 | 2.260 |
| Rmeas | 0.091 | 1.048 |
| Number of reflections | 64931 | 3316 |
| <I/σ(I)> | 10.5 | 1.7 |
| Completeness [%] | 86.1 | 47 |
| Redundancy | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 277 | 19% 2-propanol, 5% glycerol, 19%PEG4K, 0.095M Na3citrate pH5.6/HCl |
