8R8M

70S Escherichia coli ribosome with Paenilamicin B2 bound with hybrid A/P- and hybrid P/E-tRNA.

This is a non-PDB format compatible entry.

EM specimen

Vitrification

Yes

EM Data Collection

Microscope	FEI TITAN KRIOS
Electron Gun	FIELD EMISSION GUN
Accelerating Voltage (kV)	300
Detector	FEI FALCON IV (4k x 4k)

3D Reconstruction

Method	SINGLE PARTICLE
Resolution (Å)	2.4
Software	REFMAC
CTF correction	PHASE FLIPPING AND AMPLITUDE CORRECTION