8R7Z
transcription factor BARHL2 homodimer with spacing four bp
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-07-19 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.97626 |
Spacegroup name | I 21 3 |
Unit cell lengths | 159.955, 159.955, 159.955 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.000 - 3.260 |
R-factor | 0.20067 |
Rwork | 0.198 |
R-free | 0.25246 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 2.297 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHENIX |
Refinement software | REFMAC (5.8.0419) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.170 | 3.520 |
High resolution limit [Å] | 3.260 | 3.260 |
Rmerge | 0.179 | 5.142 |
Rmeas | 0.184 | 5.280 |
Rpim | 0.041 | 1.171 |
Total number of observations | 230484 | 41538 |
Number of reflections | 10697 | 2115 |
<I/σ(I)> | 8.7 | 0.7 |
Completeness [%] | 99.3 | |
Redundancy | 21.5 | 19.6 |
CC(1/2) | 0.995 | 0.204 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 0.05M Sodium Cacodilate buffer (pH 6.5), PEG (4000), PEG(200) |