8R7O

HUWE1 WWE domain in complex with 2'F-ATP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-11-15
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.999824
Spacegroup name	P 43 21 2
Unit cell lengths	62.915, 62.915, 231.945
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.640 - 1.360
R-factor	0.1693
Rwork	0.168
R-free	0.19510
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	1.229
Data reduction software	XDS ((VERSION Feb 5)
Data scaling software	autoPROC ((Version 1.1.7))
Phasing software	PHASER
Refinement software	PHENIX (1.21_5207)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	60.721	1.450
High resolution limit [Å]	1.360	1.361
Rmerge	0.060
Number of reflections	86990	4348
<I/σ(I)>	23.2
Completeness [%]	86.2	25.4
Redundancy	20
CC(1/2)	0.999	0.649

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		278	0.1 M Na Acetate pH 4.83, 2.9 M NaCl