8R7F
Transcription factor BARHL2 homodimer with spacing two bp
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-07-16 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.619920 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 45.335, 63.169, 51.323 |
| Unit cell angles | 90.00, 111.72, 90.00 |
Refinement procedure
| Resolution | 47.680 - 1.980 |
| R-factor | 0.23476 |
| Rwork | 0.233 |
| R-free | 0.27262 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 2.174 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.680 | 2.030 |
| High resolution limit [Å] | 1.980 | 1.980 |
| Rmerge | 0.083 | 3.426 |
| Rmeas | 0.092 | 3.821 |
| Rpim | 0.039 | 1.656 |
| Total number of observations | 100419 | 5704 |
| Number of reflections | 18391 | 1164 |
| <I/σ(I)> | 4.6 | 0.5 |
| Completeness [%] | 98.2 | |
| Redundancy | 5.5 | 4.9 |
| CC(1/2) | 0.997 | 0.414 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | Sodium Cacodilate buffer (pH 6.5), PEG (4000) 5% butanol |
