8R79
The D2 domain of human DTX3L
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I23 |
| Synchrotron site | Diamond |
| Beamline | I23 |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2021-10-22 |
| Detector | DECTRIS EIGER2 X 16M |
| Wavelength(s) | 2.7552 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 140.893, 140.893, 41.037 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 70.450 - 2.180 |
| R-factor | 0.21428 |
| Rwork | 0.210 |
| R-free | 0.24885 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.509 |
| Data reduction software | autoPROC |
| Data scaling software | XSCALE |
| Phasing software | HKL2Map |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 70.450 | 2.334 |
| High resolution limit [Å] | 2.180 | 2.181 |
| Rmerge | 0.107 | |
| Number of reflections | 12554 | 628 |
| <I/σ(I)> | 33.3 | 1.7 |
| Completeness [%] | 91.7 | 67.2 |
| Redundancy | 90.6 | 55.2 |
| CC(1/2) | 1.000 | 0.842 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.3 | 294 | 2.2 M (NH4)2SO4, 100 mM BisTris |
