8R74
Galectin-1 in complex with thiogalactoside derivative
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-04-19 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 53.139, 62.352, 98.016 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.715 - 1.540 |
| Rwork | 0.175 |
| R-free | 0.21890 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 2.093 |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.720 | 1.570 |
| High resolution limit [Å] | 1.540 | 1.540 |
| Rmerge | 0.054 | 0.760 |
| Number of reflections | 48869 | 15024 |
| <I/σ(I)> | 10 | |
| Completeness [%] | 99.9 | |
| Redundancy | 4.24 | |
| CC(1/2) | 0.999 | 0.510 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 288 | 0.1 M sodium acetate pH 6.2, 29 % PEG 4000, 0.2 M ammonium sulphate, seeded |
