8R6V

The complex of glycogen phosphorylase with EGCG (epigallocatechin gallate) and caffeine.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-03-14
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9763
Spacegroup name	P 43 21 2
Unit cell lengths	126.436, 126.436, 115.353
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	89.400 - 2.500
R-factor	0.16469
Rwork	0.161
R-free	0.23345
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	REFMAC (5.8.0267)
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	126.440	2.600
High resolution limit [Å]	2.500	2.500
Number of reflections	32975	3649
<I/σ(I)>	18.6	9.4
Completeness [%]	100.0
Redundancy	10.6	10.4
CC(1/2)	0.998	0.986

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	BATCH MODE	6.7	289	10mM BES buffer pH 6.7