8R6E

SARS-CoV-2 Nucleocapsid dimerization domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-11-08
Detector	DECTRIS PILATUS3 X 2M
Wavelength(s)	0.873128
Spacegroup name	P 21 21 21
Unit cell lengths	43.620, 47.372, 134.625
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.730 - 1.530
R-factor	0.22671
Rwork	0.225
R-free	0.26653
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.713
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MoRDa
Refinement software	REFMAC (5.8.0419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.370	1.550
High resolution limit [Å]	1.530	1.530
Number of reflections	43189	1968
<I/σ(I)>	5.9
Completeness [%]	99.6
Redundancy	7.1
CC(1/2)	0.997	0.291

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		297	0.2M sodium chloride, 0.1M sodium/potassium phosphate 6.5 and 25% PEG1000