8R53
The complex of Glycogen Phosphorylase with (-)-Epigallocatechin-3-gallate (EGCG) and glucose.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1) |
| Synchrotron site | PETRA III, EMBL c/o DESY |
| Beamline | P13 (MX1) |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-03-14 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9763 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 126.183, 126.183, 115.232 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 85.240 - 2.000 |
| R-factor | 0.15723 |
| Rwork | 0.155 |
| R-free | 0.19729 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.587 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | REFMAC (5.8.0267) |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 126.180 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 63292 | 4389 |
| <I/σ(I)> | 27.4 | 10.8 |
| Completeness [%] | 100.0 | |
| Redundancy | 9.3 | |
| CC(1/2) | 0.990 | 0.995 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH MODE | 6.7 | 289 | 10 mM BES buffer |
